Boar seminal plasma component in washed boar spermatozoa

Proteomics of boar seminal plasma

Influence of seminal plasma on the kinematics of boar spermatozoa during freezing.

Sperm motility is, for its relation to cell viability and fertility, a central component of the spermiogram, where consideration of motion patterns allows discrimination of sub-populations among boar spermatozoa. Extension and cryo-preservation imposes changes in these patterns in connection to handling, additives, temperature changes and the removal of boar seminal plasma (BSP) which apparentl...

Protein analysis of boar seminal plasma proteins with protective effect during low-temperature storage of spermatozoa

The present study aimed to investigate the effect of different seminal plasma proteins (SPPs) on boar spermatozoa functional characteristics. We investigated the putative protective effect of SSPs on sperm cells motility and velocity, as well as on the integrity of the plasma membrane (PM) during low-temperature storage at 4 °C. SPPs fractions were obtained and purified by gel permeation chroma...

Observations on the survival of washed boar spermatozoa in different media.

Semen, collected from boars of proven fertility, was concentrated by centrifuging the strained ejaculate at 400 g for 15 min at 20\s=deg\C and gently resuspending the sperm pellet in one-fifth the original volume of seminal plasma. The concentrated semen was then diluted 1:6 either in a chosen wash medium (see Table 1 for composition of media) and used immediately, or in a milk diluent (MFP), c...

Secretory activity of boar seminal vesicle glands.

This paper reviews the recent research on the physiological role of peptide and protein substances synthesized in boar seminal vesicle glands. Secretions from these glands are the major components of seminal plasma. Unique biochemical properties of seminal vesicle proteins, which determine their numerous functions in the reproductive processes, are discussed. The discovery of phosphotyrosine ac...